Millions of people suffer from symptoms as a result of reflux, where the contents of the stomach pass through the lower oesophageal sphincter and into the oesophagus. This can be caused by various lifestyle and diet habits, and other things such as obesity, hiatus hernia and pregnancy. Reflux can be compounded by a weakened lower oesophageal sphincter too which enables the contents of the stomach to escape through the weakened valve more easily.

In some instances, reflux episodes can reach the larynx, pharynx and nasal cavity, causing extra-gastrointestinal symptoms (see below).

Left untreated, prolonged exposure of the different mucosa to the contents of reflux, the disorder can lead to Barrett’s oesophagus, oesophageal adenocarcinoma (cancer) and laryngeal cancer.

Symptoms of Reflux

Gastroenterological Symptoms of Reflux:

  • Heartburn

  • Difficulty swallowing (dysphagia)

  • Nausea and regurgitation

  • Vomiting

Ear, Nose and Throat (ENT) Symptoms of Reflux:

  • Sore throat or hoarseness

  • Chronic cough or persistent throat congestion

  • Asthma

  • Post nasal drip

  • Difficulty swallowing

Diagnose Reflux with Peptest™

Until recently, reflux diagnosis involved complex and expensive procedures including 24/48 hour pH monitoring, pH Impedance testing, Barium meal and gastroscopy.

Peptest is a simple, effective and low cost test that tells you conclusively that a patient has reflux. By measuring Pepsin in saliva samples, it is a clear indicator that a reflux episode has occurred because pepsin is only produced in the stomach. If it is detected in the oesophagus, throat, mouth or lungs it identifies reflux and enables a swift and appropriate treatment plan to be made.

Diagnose difficult symptoms

Monitor disease activity

Select appropriate


For GP and Specialist use

BIOHIT HealthCare in Reflux and Dyspepsia 

Millions of people in the UK and worldwide suffer from non-specific gastroesophageal symptoms which are challenging for the treating physician to manage. BIOHIT HealthCare has academic interest in dyspepsia as well as a commercial role in supplying innovative test systems to help physicians diagnose and monitor dyspepsia and associated diseases.


BIOHIT HealthCare supplies essential tests that help investigate dyspepsia in Primary and Secondary Care, helping treating physicians to adopt a test-and -treat or test-and-refer strategy to improve their practice. With BIOHIT's non-invasive GastroPanel and Peptest diagnostic tests, the treating physician can determine the health and function of the stomach prior to treating, and, gain a clear understanding of the involvement of acid and pepsin. The tests help physicians decide whether the patient should be referred to Secondary Care for further investigation or treated in Primary Care with confidence.

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Kit for Labs

Set up Peptest in your own laboratory with ease

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Peptest™ Service Laboratory

Send samples to our service laboratory for Peptest analysis

Should the patient pause their PPI, H2RA, or antacid therapy for the test?

Saliva samples that are sent to our Peptest laboratory for analysis are analysed upon receipt and you can expect your results to be sent to you within two to three days. Results are provided in a report that indicates whether each individual sample is positive or negative. Positive samples are quantified enabling treatment to be adjusted according to the concentration of Pepsin found.

What is the laboratory turnaround time?

Collecting samples at the right time ensures that the Peptest delivers the best results. Like with any diagnostic test, sample quality is crucial to its performance. Studies have shown that by taking three samples at the optimum time, users can receive results of the highest accuracy. Three samples also enables you to determine whether the patient is susceptible to experiencing reflux episodes when lying down which in turn can help you manage your patient's symptoms more effectively.

Why do my patients need to submit 3 samples?

The performance of Peptest has been validated in by many clinical studies which show that Peptest is highly accurate:

Sensitivity: 95%
Specificity: 89%
Pepsin detection limit: 16 nanograms per millmetre (16ng/ml)


By determining the concentration of pepsin in a patient's saliva it accurately diagnoses, and indicates the severity of reflux. Peptest can therefore also be used to guide therapy and to evaluate treatment success.

What is the accuracy of Peptest?

Frequently asked Questions

It is recommended that patients stop taking antacids and raft-forming/ alginate suspensions (e.g. Gaviscon®) for 48 hours prior to collecting samples for Peptest analysis. Patients do not need to pause PPI or H2 receptor antagonist therapy as Peptest will still detect pepsin in the presence of these medications if pepsin is being refluxed.

For laryngopharangeal reflux (LPR) symptoms Peptest can be a useful tool to help investigate the cause. When a patient presents with symptoms of LPR a positive pepsin result this indicates that reflux has occurred and the reflux can be treated appropriately. Conversely, if Peptest is negative when testing patients with LPR symptoms, this can be more difficult to manage, though it is useful to rule out Pepsin as the culprit. In such cases, the physician can look to alternative explanations such as bacterial colonisation or previous events that have inflamed or caused the epithelia to become more sensitive to lifestyle and environmental factors. Negative Peptest results may also indicate the need to investigate allergies and re-evaluate lifestyle characteristics of the patient.

Can Peptest be used in the investigation of laryngopharangeal reflux (LPR)?