IDKmonitor Adalimumab TOTAL Anti-drug antibody ELISA 96 wells

Product family:
Product Code:
Therapeutic Drug Monitoring
K9651

Product Information:

Adalimumab is a recombinant protein that has the potential to induce humoral immune responses and lead to anti-drug antibody formation; a major cause of loss of response.

 

These Anti-drug antibodies bind and neutralise the TNFα inhibitors accelerating their clearance and reduces the efficacy of the drugs.  Monitoring the efficacy of TNFα inhibitors may help clinicians schedule a successful individualised treatment strategy for their patients.

Total Anti-Adalimumab Antibody ELISA

TOTAL anti-drug antibody ELISAs enable the detection of anti-drug antibodies in the presence of circulating drug, and so are often called "Drug Tolerant" assays. The benefit of drug-tolerant assays is being able to detect lower titres of Anti-drug antibodies which could become clinically significant in certain circumstances.

Total anti-drug Antibody assays offer a greater sensitivity, unmasking the drug-bound portion of anti-drug antibodies in circulation. TOTAL anti-drug antibody analysis may be advantageous when using combination therapy such as immunosuppressants due to this increased sensitivity.

IDKmonitor® Adalimumab total-Anti-drug Antibody ELISA is a quantitative assay that involves a pre-analytical step to dissociate the anti-drug antibodies of interest from the therapeutic Adalimumab. The separated therapeutic Adalimumab is replaced by a biotinylated version of the drug, forming a the new complex which can be captured and quantified on the streptavidin coated microplate.

Features:

  • Drug tolerant assay for Adalimumab anti-drug antibodies

  • Detects Total anti-Adalimumab antibodies in Serum and Plasma

  • Preanalytical step to dissociate therapeutic drug from target anti-drug antibody

  • 1 calibrator, 2 controls

  • Capacity for 45 samples in duplicate

  • Automatable on ELISA automates (e.g. Dynex D2)

Benefits:

  • Supports the investigation of primary non response and loss of response to Adalimumab

  • Drug tolerant assay detects total anti-Adalimumab antibodies (those free- and bound to- Adalimumab)

  • Helps predict long term outcome of disease in patients with sub-therapeutic Adalimumab levels

  • Identifies patients with low-titre anti-Adalimumab antibodies (e.g. in the case of concomitant immunosuppressant)

  • UK publications and evidence base

  • Widely available via reference laboratories in UK and Ireland

  • Applicable to Gastroenterology, Rheumatology

Format

ELISA

Storage (°C)

+2 to +8

Sample type

Blood

Category

Laboratory

Index

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BIOHIT HealthCare Ltd

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North Road

Ellesmere Port

Cheshire CH65 1AD

United Kingdom

Tel: +44 151 550 4 550

Fax: +44 151 550 4 551

cs@biohithealthcare.co.uk

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