IDK Zonulin (Stool) ELISA 96 wells

Product family:
Product Code:
Faecal Tests
K5600

New

Product Information:

The IDK Zonulin ELISA is a quantitative assay for the determination of zonulin in stool samples. 

 

The assay is a competitive ELISA. Biotinylated zonulin tracer is used to treat extracted stool samples, standards and controls before being incubated in microplate coated with polyclonal anti-zonulin antibodies.

During the incubation, the free target antigen in the samples competes with the biotinylated zonulin tracer for the binding of the polyclonal anti-zonulin antibodies immobilised on the microplate. Peroxidase-labeled streptavidin is then added which binds to the biotinylated zonulin. When tetramethylbenzidine substrate is added to the complex the resultant colour change is inversely proportional to the concentration of Zonulin in the sample.

Features:

  • Measures the concentration of Zonulin in stool samples

  • Simple competitive ELISA technology

  • Support for both automated and manual analysis methods

  • 5 calibrators

  • 2 controls

  • Capacity for 41 samples in duplicate

  • Total incubation time 130 minutes

  • Easily Automated on ELISA automate (e.g. Dynex DS2)

Benefits:

  • One stool extract can be used in multiple assays (e.g. Zonulin, Calprotectin, Lactoferrin, Pancreatic Elastase, FIT, Faecal Bile Acids)

Format

ELISA

Storage (°C)

+2 to +8

Sample type

Stool

Category

Laboratory

Index

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BIOHIT HealthCare Ltd

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North Road

Ellesmere Port

Cheshire CH65 1AD

United Kingdom

Tel: +44 151 550 4 550

Fax: +44 151 550 4 551

cs@biohithealthcare.co.uk

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