IDK Faecal Lactoferrin ELISA 96 wells

Product family:
Product Code:
Faecal Tests
K6870

Product Information:

The IDK Lactoferrin ELISA is an enzyme immunoassay for the quantitative determination of Lactoferrin in stool. The assay utilises the sandwich technique with antibodies that bind to Lactoferrin.

Standards, controls and diluted patient samples which are assayed for lactoferrin are added to wells of the microplate which is coated with a polyclonal antibody. A complex is formed by adding a peroxidase labelled conjugate to each well followed by a TMB substrate.

The method is simple, fast and reliable with a total incubation time of just 70 minutes and can be performed as a manual ELISA or automated on any open ELISA automate (e.g. Dynex DS2).

Stool Sample Extraction

Homogenised stools are extracted using IDK Extract® - the universal Faecal Extraction buffer from Immundiagnostik that can be used in a multitude of faecal assays. The extraction process is fast, clean and precise using the rapid Stool Sample Application System (SAS) to prepare a 1:100 dilution which can be directly loaded on to automated platforms for analysis. The extracts are further diluted on-board 1:10 for assaying.

Features:

  • Measures the concentration of Lactoferrin in stool samples

  • Simple sandwich ELISA technology

  • Support for both automated and manual analysis methods

  • 5 calibrators

  • 2 controls

  • Capacity for 41 samples in duplicate

  • Total incubation time 70 minutes

  • Easily Automated on ELISA automate (e.g. Dynex DS2)

Benefits:

  • One stool extract can be used in multiple assays (e.g. Lactoferrin, Calprotectin, Zonulin, Pancreatic Elastase, FIT, Faecal Bile Acids)

Format

ELISA

Storage (°C)

+2 to +8

Sample type

Stool

Category

Laboratory

Index

© 2018 BIOHIT HealthCare Ltd

Contact Us

BIOHIT HealthCare Ltd

Pioneer House, Pioneer Business Park

North Road

Ellesmere Port

Cheshire CH65 1AD

United Kingdom

Tel: +44 151 550 4 550

Fax: +44 151 550 4 551

cs@biohithealthcare.co.uk

  • Twitter Social Icon