IDK Faecal Lactoferrin ELISA 96 wells
The IDK Lactoferrin ELISA is an enzyme immunoassay for the quantitative determination of Lactoferrin in stool. The assay utilises the sandwich technique with antibodies that bind to Lactoferrin.
Standards, controls and diluted patient samples which are assayed for lactoferrin are added to wells of the microplate which is coated with a polyclonal antibody. A complex is formed by adding a peroxidase labelled conjugate to each well followed by a TMB substrate.
The method is simple, fast and reliable with a total incubation time of just 70 minutes and can be performed as a manual ELISA or automated on any open ELISA automate (e.g. Dynex DS2).
Stool Sample Extraction
Homogenised stools are extracted using IDK Extract® - the universal Faecal Extraction buffer from Immundiagnostik that can be used in a multitude of faecal assays. The extraction process is fast, clean and precise using the rapid Stool Sample Application System (SAS) to prepare a 1:100 dilution which can be directly loaded on to automated platforms for analysis. The extracts are further diluted on-board 1:10 for assaying.
Measures the concentration of Lactoferrin in stool samples
Simple sandwich ELISA technology
Support for both automated and manual analysis methods
Capacity for 41 samples in duplicate
Total incubation time 70 minutes
Easily Automated on ELISA automate (e.g. Dynex DS2)
One stool extract can be used in multiple assays (e.g. Lactoferrin, Calprotectin, Zonulin, Pancreatic Elastase, FIT, Faecal Bile Acids)
+2 to +8