IDK Faecal Haemoglobin ELISA 96 wells
The IDK Faecal Haemoglobin ELISA is an enzyme immunoassay for the quantitative determination of human haemoglobin in stool. The assay utilises the two-site sandwich technique with antibodies that bind to human haemoglobin.
Standards, controls and diluted patient samples which are assayed for faecal haemoglobin are added to wells of the microplate which is coated with anti-human haemoglobin antibody. A complex is formed by adding a peroxidase labelled conjugate to each well followed by a TMB substrate.
The method is simple, fast and reliable with a total incubation time of just 130 minutes and can be performed as a manual ELISA or automated on any open ELISA automate (e.g. Dynex DS2).
Stool Sample Extraction
Homogenised stools are extracted using IDK Extract® - the universal Faecal Extraction buffer from Immundiagnostik that can be used in a multitude of faecal assays. The extraction process is fast, clean and precise using the rapid Stool Sample Application System (SAS) to prepare a 1:100 dilution which can be directly loaded on to automated platforms ready for analysis.
Stool extracts are stable at room temperature (15-30°C), 2–8°C, and -20°C for 7 days.
Highly specific FIT test in sandwich ELISA format
Capacity for 41 samples in duplicate
Total incubation time 130 minutes
Broad linear range (up to 50µg/g)
IDK Extract® universal stool extraction buffer
Rapid Extraction Tubes (Stool Sample Application System)
Easily Automated on ELISA automate (e.g. Dynex DS2)
Highly specific for Human Haemoglobin
7 day sample stability at Room Temperature with IDK Extract sample extraction buffer
Fast, high throughput ELISA
No cross-reactivity with Pancreatic amylase, MPO, Lysozyme, Chymotrypsin, sIgA, Albumin
+2 to +8