EDI™ Novel Coronavirus COVID-19 IgM ELISA Kit
Covid-19 / SARS-CoV-2
EDI™ Novel Coronavirus COVID-19 IgM ELISA Kit is for the qualitative measurement of the COVID-19 IgM antibody in serum. This assay utilizes the “IgM capture” method on microplate based enzyme immunoassay technique. Assay controls and samples are added to the microtiter wells of a microplate that is coated with a anti-human IgM specific antibody.
After the first incubation period, the unbound protein matrix is removed with a subsequent washing step. A horseradish peroxidase (HRP) labeled recombinant COVID-19 antigen is added to each well. After an incubation period, an immunocomplex of "Anti-hIgM antibody COVID-19 IgM antibody - HRP labeled COVID-19 antigen" is formed if there is novel coronavirus IgM antibody present in the tested materials.
The unbound tracer antigen is removed by the subsequent washing step. HRP-labeled COVID-19 antigen tracer bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antigen bound to the coronavirus IgM on the wall of the microtiter well is proportional to the amount of the coronavirus IgM antibody level in the tested materials.
96 well plate ELISA
Qualitative detection of human IgM antibodies against SARS-CoV-2 in serum
Contains positive and negative controls
Total incubation time 80 mins
Automatable or manual
- Specific detection of IgM Antibodies against SARS-CoV-2 (COVID-19) antigen
- Suitable for Screening, detection of early phase immune response to SARS-CoV-2, evidence of COVID-19 infection
+2 to +8