Biohit Active B12 (Holotranscobalamin), 96 wells

Product family:
Product Code:
Vitamins and Nutrition Assays

Product Information:

Biohit Active B12 (holotranscoblamin) ELISA  - an accurate test for measuring the levels of active vitamin B12


Traditional methods of diagnosing vitamin B12 deficiency involved measuring the concentration of total vitamin-B12 in serum. The total vitamin B12 concentration essentially reflects vitamin-B12 which is bound to its two carrier proteins forming, holohaptocorrin (holoHC) and holotranscobalamin(holoTC). Whilst holoHC accounts for 70% - 80% of the vitamin B12 in serum, only holoTC (active vitamin B12) can be used by human cells. Measurement of total vitamin B12 can hence give erroneous results because it measures the vitamin B12 which is in circulation but does not indicate the active vitamin B12 that is available to the cells of the body.


BIOHIT Active B12 test provides a solution to this diagnostic paradox: this test directly measures (holoTC) – the biochemically active form of vitamin B12 – from the human serum. This test is well suited for the screening of patients with a suspected vitamin B12 deficiency. BIOHIT Active B12 test can also be used for confirming the vitamin B12 status in the large number of patients who get an inconclusive result from total vitamin B12 tests.


Product specifications


  • Measures the concentration of active vitamin B12 (holotranscobalamin) available to the cells

  • Proven ELISA technology, support for both automated and manual analysis methods

  • Unlike total B12 kits, no issues with Intrinsic Factor Blocking Antibody (IFBA) interference

  • Numerous clinical studies proving the performance of Active B12 over total B12


  • Measures the concentration of active vitamin B12 (holotranscobalamin) available to the cells

  • Simple ELISA technology

  • Support for both automated and manual analysis methods

  • No interference from Intrinsic Factor Blocking Antibody (IFBA)


  • Provides detailed information about the active form of Vitamin B12 at a cellular level

  • Effective for resolving indeterminate Total Vitamin B12 values

  • Open ELISA format ideally suited to any laboratory



Storage (°C)

+2 to +8

Sample type



Laboratory / Service Lab


Contact Us

BIOHIT HealthCare Ltd

Pioneer House, Pioneer Business Park

North Road

Ellesmere Port

Cheshire CH65 1AD

United Kingdom

Tel: +44 151 550 4 550

Fax: +44 151 550 4 551

© 2020 BIOHIT HealthCare Ltd

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